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KMID : 0613820130230081019
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Journal of Life Science
2013 Volume.23 No. 8 p.1019 ~ p.1024
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Anti-oxidative Activity and the Protective Effect of Donkey's Bone and Skin Extracts on SK-N-SH Cells
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Kim Dong-wook
Chae Hyun-Seok
Kim Nam-Young
Jang Ae-Ra
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Abstract
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The aims of this study were to determine antioxidation effect and neuroblastoma cell protection effect of donkey's bone and skin extracts (DBSE). DBSE was extracted by a pressure-cooker for 48 h and lyophilized. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was significantly increased with increased doses of DBSE and 40 mg/ml of DBSE showed 95.43% of the DPPH scavenging effect, which was equivalent to 1 mg/ml of vitamin C. The 2,2'-azino-bis (3-ethylbenzothiazoline-6- sulphonic acid) (ABTS) radical scavenging activity was also increased in a dose-dependent manner, and 20 mg/ml of DBSE showed 88.73% of the ABTS scavenging effect. The oxygen radical absorbance capacity (μM Trolox equivalent) of DBSE was significantly increased at a concentration of 10 mg/ml, which showed 132.53μM TE. The viability of oxidatively stressed brain cells induced by 500μM H₂O₂ was protected by DBSE at concentrations greater than 50μM. Cell viability after DBSE treatment at 50 and 100μg/ml was 53.78 and 54.34μM TE, respectively. There was no significant difference between both doses; however, 200 and 500μg/ml of DBSE showed 59.74 and 66.08% of cell viability, respectively indicating that DBSE protected SK-N-SH from oxidation stress. These results suggest that DBSE may have potential to be used as natural antioxidants in food industry, while in vivo evidence is necessary to support DBSE's in vitro-based antioxidative efficiency.
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KEYWORD
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Donkey, bone and skin, antioxidative, SK-N-SH, neuroblastoma
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